BioFlo 320

//BioFlo 320
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BioFlo 320

The BioFlo 320, next-generation bioprocess control station, is designed as a universal platform capable of meeting the ever changing needs of all segments of the biotech and pharmaceutical sciences. Suitable for microbial to cell culture, scale up to scale down, or batch to fed-batch process, the BioFlo 320 has the right combination of features to get the job done, all within an ultra-compact footprint.


Product Description

Product Information

The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition. Extensive options give you the customizable solution your lab requires. Combined with the strict quality standards that all Eppendorf products are held to, the BioFlo 320 truly is the new premium choice in benchtop bioprocess systems.

Small footprint… big impact:
From R&D laboratories to pilot-scale production facilities, space is a major factor when selecting the right equipment. The BioFlo 320 offers flexibility, better control, and maximum functionality while occupying a fraction of the valuable lab space of similar systems. This means greater efficiency and productivity at a lower operating cost for your lab.

Intelligent Sensors

› Integrated Mettler Toledo Intelligent Sensor Management (ISM) platform
› Universal connections for up to four analog or digital (ISM) sensors
› pH: analog or digital (ISM)
› DO: analog or digital (ISM)
› Optical DO: digital (ISM)
› Redox: analog or digital (ISM)
› CO2: digital (ISM)
› Real-time sensor diagnostics anticipate sensor failure

Advanced Software Solutions

› Control eight units from a single user interface
› Automatic gas mixing algorithms for simplified control (4-gas, 3-gas)
› O2 enrichment, N2 enrichment)
› New ten-point cascade feature for sophisticated control strategies
› Built-in elapsed fermentation timer for batch management
› Trend display with up to twelve process values within a single view
› Remote access via PC or tablet


  • Interchangeable autoclavable and BioBLU® Single-Use Vessels
  • Integrated Mettler Toledo® Intelligent Sensor Management (ISM) platform
  • Control up to eight systems from a single user interface
  • Universal control for both microbial and cell culture applications
  • Field-upgradable TMFC drawers for sparge and overlay gas
  • Enhanced software package with new cascade and time profile features
  • Built-in optical pH sensing technology for use with the BioBLU Single-Use Vessels
  • Ethernet communication for multi-unit control, Eppendorf SCADA software, and IP addressing
  • Up to six integrated pumps capable of operating in variable-speed mode
  • Eight independently controlled process gas supplies


  • Research and development in cell culture and microbiology
  • Bench- and pilot-scale fermentation of aerobic and anaerobic bacteria, yeasts, and fungi
  • Cultivation of mammalian, insect, and human cell lines
  • Specialized applications such as stem cell culture or biofeul/biopolymer development
  • Specialized packed-bed impeller for vaccine production in anchorage and non-anchorage dependent cell lines
  • Suitable for batch, fed-batch, and continuous processes
  • Validation packages available for GMP-regulated processes

Technical Data

BioFlo® 320
Bacteria/Yeast/Fungi yes
Insect yes
Mammalian/Animal yes
Plant/Algae yes
Stem cells yes
Number of parallel units Up to 8
Power supply 100 – 240 V, 50/60 Hz
Dimensions (W × D × H) 40.6 × 40.6 × 66.0 cm / 16 × 16 × 26 in
Vessels Glass/single-use vessels


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