Brewster Angle Microscopes (BAM) enable visualization of monolayers, typically at the air-water interface in a Langmuir Trough. They create an image of the surface by detecting changes in the refractive index of the water surface in the presence of surfactant molecules. This provides information on homogeneity, phase behavior and film morphology.
Brewster Angle Microscope
KSV NIMA BAM
- The best resolution (2 µm) and image quality in the field of Brewster Angle Microscopy and provides real-time and fully focused sample visualization at 20-35 fps .
- Easy integration with KSV NIMA L & LB Trough Large.
- Advanced image analysis and processing with the ability to simply select domains to get their dimensions .
- Adjustable incident angle (52-57°) enabling measurements with dielectric solid substrates like glass or quartz .
- Imaging of anisotropic layers with the motorized analyzer .
- On-line automatic subtraction of a constant background image .
- Possible desktop remote control for support purposes.
KSV NIMA MicroBAM
- Appropriate resolution (12 µm) for most applications .
- Capture and save still images and real-time video footage of monolayers.
- Compact design, small footprint .
- Simple, intuitive operation .
- Imaging and control software included .
- Operates with a PC or Notebook (Windows XP to Win 7) .
- Compatible with most Langmuir troughs and Langmuir-Blodgett troughs .
Brewster Angle Microscopes (BAM) enable the visualization of Langmuir monolayers or adsorbate films at the air-water interface. In conjunction with a Langmuir Trough it is possible to study:
- Monolayer/film behavior. It is possible to observe phase changes, phase separation, domain size, shape and packing.
- Monolayer/film homogeneity. When combined with a KSV NIMA L & LB Trough, observation can be performed during compression/expansion at known surface pressures.
- Influence of sub-phase conditions on film structures. Observe and study monolayer/film behavior and formation in different sub-phase conditions including salt concentrations, pH and temperature etc.
- Monitoring of surface reactions. For example, photochemical reactions, polymerization reactions as well as enzyme kinetics can be followed in real-time.
- Monitoring and detection of surface active materials. For example protein adsorption and nanoparticle flotation. BAMs are primarily designed for the air-water interface. However under some conditions the KSV NIMA BAM can be used for other interfaces such as air-glass.
KSV NIMA offers two different Brewster Angle Microscopes, the advanced KSV NIMA BAM and the compact KSV NIMA MicroBAM.
KSV NIMA BAM
The KSV NIMA BAM represents the latest in BAM instrumentation, allowing high resolution and fully focused real-time imaging of monolayers. The KSV NIMA BAM provides completely undistorted images unlike other BAMs. A precise motorized vertical lift allows fine positioning to focus the water surface. An active isolation system is integrated to the KSV NIMA BAM to eliminate disturbing environmental vibration from air conditioning, traffic etc.
A high performance camera and dedicated calibration algorithms allow quantitative measurements of reflectivity for monitoring adsorption kinetics or thickness variation. The KSV NIMA BAM is equipped with a motorized analyzer to visualize optical anisotropy due to long-range molecular orientation order in monolayers.
The software offers advanced and comprehensive image analysis and processing functionalities. A Langmuir trough can be used together with the KSV NIMA BAM for control over the monolayer packing density and recording of surface pressure. The compatible KSV NIMA Langmuir and Langmuir-Blodgett Deposition Trough Large are the recommended systems. A PC is included with the instrument.
KSV NIMA BAM
KSV NIMA MicroBAM
The KSV NIMA MicroBAM is easy-to-use, entry level instruments for non-invasive imaging of mono-molecular layers at the air-water interface. The excellent image quality and good lateral resolution make them ideal instruments for the visualization of morphological film parameters (e.g. compressed film homogeneity, domain size, shape and packing). Real-time observation and recording of film structure enables dynamic activity to be captured.
The KSV NIMA MicroBAM can be used with most KSV NIMA Langmuir and Langmuir-Blodgett Troughs, to provide real-time image measurements as a function of time or surface pressure. The KSV NIMA MicroBAM connects directly to the computer via USB making it remarkably easy to setup and use. The instrument has a safety key interlock for the BAM laser.
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