As a life scientist, you want to see how biological materials look like at nanoscale resolution and how soft they are in liquid and buffer conditions. Park NX-Bio enables that with its innovative in-liquid imaging Scanning Ion Conductance Microscopy (SICM) and its highly acclaimed Atomic Force Microscopy (AFM) technology.
Park NX-Bio is a powerful 3-in-1 bio-research tool that uniquely combines SICM with AFM and an inverted optical microscope (IOM) on the same platform. The modular design of the Park NX-Bio allows researchers to easily switch between its SICM and AFM capabilities. Designed for non-invasive in-liquid imaging, Park NX-Bio is the ideal tool for studying biological materials under physiological conditions. It combines the bio-mechanical property measurement capability of the AFM and nano imaging of the SICM in liquid, and the optical viewing of the IOM.
Park NX-Bio has a user-friendly design and automated imaging software for SICM, so you won’t have to spend so much time for in-liquid imaging. The basic setup for operation can be learned through a simple training course in only a few hours. This allows you to quickly shift your time to conducting more advanced research for your subject.
Park SICM can acquire biological images at nanoscale in physiological conditions, attaining high resolution of less than 200 nm. The biological images obtained from SICM are free from morphological deformation, which can occur from scanning electron microscopy (SEM) or even AFM systems.
In Scanning Ion Conductance Microscopy developed by Park Systems (Park SICM), a glass nanopipette filled with an electrolyte acts as an ion sensor that provides feedback on its location relative to a sample completely immersed in liquid. The pipette tip maintains its distance from the sample by keeping the ionic current constant. In comparison, AFM typically relies on interaction of forces between its probe tip and the sample.
AFM uses a micro-thin cantilever and tip as a probe. For Park SICM, the pipette is a probe with an inner diameter ranging from 80 to 100 nanometers for pipettes made of glass and 30-50 nanometers for those made of quartz.
Similar to Scanning Tunneling Microscopy (STM) operating in ambient air, the Park SICM operates in liquid without making physical contact with the sample. Electrodes on either side of the sample and pipette produce ionic current that flows through the surrounding solution. A sensor measures the current flow, which decreases as the distance between the pipette and sample becomes smaller, and monitors the distance between the pipette and the sample to obtain the topology.
Force-distance (FD) spectroscopy using an AFM is a beneficial tool to characterize bio-mechanical properties of various biological materials. In FD spectroscopy, the cantilever tip touches the sample surface with a user prescribed amount of force accurately applied using the AFM’s Z scanner. Park AFM’s industry leading low noise Z detector allows the researcher to control Z scanner movement to apply an exact amount of force very accurately to a sample surface during the FD spectroscopy. This enables the researcher to collect detailed bio-mechanical characterization data at the nano-newton scale.
International Nano-Conference(ICN+T), Basel (CH), 2006, Noemi Rozlosnik Technical University of Denmark
The Herzian and Oliver Pharr models are calculated automatically from the Park AFM’s accurate FD spectroscopy data to determine the elastic modulus (Young’s modulus). Both of these calculation methods are included in Park XEI, the data analysis software in Park NX-Bio. They strengthen the biomechanical data verification of FD curves obtained in your experiments.
Park NX-Bio combines Park SICM’s ability to interpret morphology under true physiological conditions and Park AFM’s capacity to acquire bio-mechanical property data (elastic modulus) accurately. This enables researchers to understand the fundamentals of their biological materials at a deeper level.
• Flexure-guided structure driven by multiply-stacked piezoelectric stacks
• Z scan range: 25 μm
• 20-bit Z position control and 24-bit Z position sensor
• Automatically connects to the electronics upon mounting
• Flexure-guided structure driven by multiply-stacked piezoelectric stacks
• Z scan range: 25 μm
• 20-bit Z position control and 24-bit Z position sensor
• NCM oscillation frequency: Up to 3 MHz
• Voltage bias range to the cantilever: -10 V to 10 V
• SLD wavelength: 830 nm
• SLD has low coherence length eliminating optical interference
• SLD coherent length: ~50 μm
• Automatically connects to the electronics upon mounting
• DC mode
• ARS mode
• Z servo ARS mode
• Current-Distance (I-D) Spectroscopy
• Patch Clamping Integration (Targeted Patch Clamping)
• Force Distance (F-D) Spectroscopy
• PinPoint modeTM for Surface Mechanical Property Imaging
• Force Volume Imaging
• Spring Constant Calibration by Thermal Method
• True Non-Contact AFM
• Basic Contact AFM and DFM
• Lateral Force Microscopy (LFM)
• Phase Imaging
• Raman Spectroscopy Integration
• Tip-Enahnced Raman Spectroscopy (TERS) Integration
Dedicated system control and data acquisition software
Adjusting feedback gain, set point in real time
Script-level control through external programs such as LabVIEW (optional)
SICM & AFM data analysis software (running on Windows, Mac OS X, and Linux)
Geographical morphology of biological sample analysis, including height, volume, and surface roughness
3 dimensional SICM/AFM image display
Intel® Core™ i3 or compatible
4 GB RAM, 500 GB Hard Disc Drive
Dual 23 inch LED monitors (1920 × 1080 pixels, DVI)
Graphic Card: ATI Radeon 3450 graphics card or compatible
Operating System: Microsoft Windows 7 Professional 32 bit (English)
Decoupled XY and Z-scanner
Single module flexure XY-scanner with closed-loop control
Scan range of XY-scanner: 100 μm x 100 μm
20-bit XY position control and 24-bit XY positioning sensor
Working distance of Z-scanner: 25 μm
Resonance frequency of Z-scanner: 5 kHz
Working range of XY stage: Software-controlled motorized stage for SICM/AFM head positioning
Stage travel range: 14 mm
Stage travel step: 0.1 μm
Working range of Z stage: -14 mm, motorized movement
Sample size:
• 50 mm × 50 mm, 20 mm thick, and up to 500 g
• Petri dish (38 mm)
Compatible with inverted microcopes from
• Zeiss (Axio Observer Z.1)
• Nikon (Ti-S, Ti-U, Ti-E)
Compatible with confocal microscopes and fluorescence technique such as TIRF, STORM
TopviewOptics (upright optics) with CCD camera for opaque samples
Controls temperature, humidity, and pH
Temperature control
• Range: RT – 45 °C
• Heating elements placed at the top and bottom of the
chamber to minimize temperature fluctuation
Includes Temperature Controller and Humidifier
Includes covers for AFM head and SICM head
Controls the pH of the Live Cell Chamber by supplying mixed CO2 gas
Open/closed-cell environment for liquid imaging
Temperature control range:
0 °C to 110 °C (without liquid), 4 °C to 70 °C (with liquid)
Enhanced Acoustic Enclosure (AE) for NX-Bio
Designed exclusively for the NX-Bio, the Integrated Acoustic Enclosure for SICM/AFM isolates
the systems from external acoustic and light noise as well as floor vibration for ultimate performance.
Includes active vibration isolation system with direct velocity feedback to cancel out the floor vibration
Active frequency: 0.7 Hz to 1 kHz
Best solution for high resolution in-liquid imaging
Ergonomic design for a convenient access to the instrument
Dimension: 1,000 × 1,030 × 1,460 mm (outer)
Weight: 661 kg
0 °C to 110 °C (without liquid), 4 °C to 70 °C (with liquid)
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